30-minute genome

[groovychk]

New Scientist Health, Dec. 1, 2008

Pacific BioSciences says that by 2013, it could sequence a person's entire genome in half an hour with 99.999 per cent accuracy for under $1000.

The new technique involves attaching a different coloured fluorescent dye to each of the four types of nucleotide and watching these flash as they are incorporated into the strand (see diagram). The sequence of coloured flashes in this molecular fireworks display indicates the order in which the nucleotides appear.

the company hopes will hit the market in 2010. By 2013, it aims to squeeze a million ZMWs onto a single chip and observe DNA being assembled in each simultaneously. Company founder Stephen Turner estimates that such a chip would be able to sequence an entire human genome in under half an hour to 99.999 per cent accuracy for under $1000.

http://www.newscientist.com/article/mg20026846.800-molecular-fireworks-could-produce-30minute-genomes.html